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MOB1 Antibody

MOB1 Antibody #3863

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Product Specifications

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	24
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

MOB1 Antibody detects endogenous levels of total MOB1 protein (MOB1α and MOB1β).

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro15 of human MOB1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MOB1 was first identified in yeast as a protein that binds to Mps with essential roles in the completion of mitosis and the maintenance of ploidy (1). Its Drosophila and mammalian homologs, Mats and MOB1, respectively, are involved in the Hippo signaling tumor suppressor pathway, which plays a critical role in organ size regulation and which has been implicated in cancer development (2-5). There are two MOB1 proteins in humans, MOB1A and MOB1B, that are encoded by two different genes but which have greater than 95% amino acid sequence identity (6). Both forms bind to members of the nuclear Dbf2-related (NDR) kinases, such as LATS1/2 and NDR1/2, thereby stimulating kinase activity (7-9). This binding is promoted by the phosphorylation of MOB1 at several threonine residues (e.g., Thr12, Thr35) by MST1 and/or MST2 (5,10).

Alternate Names

C2orf6; FLJ10788; FLJ11595; MATS1; MATS2; MGC33910; MOB kinase activator 1A; MOB kinase activator 1B; MOB1; Mob1 alpha; Mob1 homolog 1A; Mob1 homolog 1B; MOB1 Mps One Binder homolog A; MOB1 Mps One Binder homolog B; MOB1, Mps One Binder kinase activator-like 1A; MOB1, Mps One Binder kinase activator-like 1A (yeast); MOB1, Mps One Binder kinase activator-like 1B; MOB1, Mps One Binder kinase activator-like 1B (yeast); Mob1A; Mob1B; MOB4A; MOB4B; MOBK1B; MOBKL1A; MOBKL1B; MOL1A; MOL1B; Mps one binder kinase activator-like 1A; Mps one binder kinase activator-like 1B; Protein Mob4A; Protein Mob4B

Database Links

UniProt ID: Q9H8S9 , Q7L9L4

Entrez-Gene Id: 55233 , 92597

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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