mNeonGreen Tag Antibody #53061
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Inquiry Info. # 53061
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Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
mNeonGreen Tag Antibody detects exogenously expressed mNeonGreen protein. This antibody does not cross-react with green flurorescent protein (GFP).
Species Reactivity:
All Species Expected
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mNeonGreen protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
mNeonGreen is a 220 amino acid, bright, green fluorescent protein derived from Branchiostoma lanceolatum. mNeonGreen is a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization (1).
mNeonGreen is a 220 amino acid, bright, green fluorescent protein derived from Branchiostoma lanceolatum. mNeonGreen is a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization (1).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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