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MCP-8 (F6M5L) Rabbit Monoclonal Antibody #40229
Filter:
- WB
- IF
Product Specifications
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40-50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
实验步骤
Specificity / Sensitivity
MCP-8 (F6M5L) Rabbit Monoclonal Antibody recognizes endogenous levels of total MCP-8 protein. This antibody detects a 90 kDa protein of unknown identity in some cell lines. Nonspecific staining has been observed in mouse neurons by immunofluorescence.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse MCP-8 protein.
Background
Mast cell protease 8 (MCP-8) is a serine protease expressed in mice and rats (1,2). Its enzymatic function has not been well characterized; however, it has been shown to induce an inflammatory response by indirectly recruiting leukocytes in a COX-mediated manner (3). Despite its name, MCP-8 is primarily expressed in mouse basophils and is often used as a phenotyping marker (4,5). Although basophils are the major mature cell population expressing the protein, MCP-8 is also transiently expressed by granulocyte-macrophage progenitors. High-dose diphtheria toxin (DT) injection into MCPT8-DTR thus reduces eosinophils, neutrophils, and basophils (6,7).
- Lützelschwab C et al. (1998) Eur J Immunol 28, 1022-33.
- Lunderius C and Hellman L (2001) Immunogenetics 53, 225-32.
- Tsutsui H et al. (2017) J Biol Chem 292, 1061-1067.
- Pellefigues C et al. (2019) Front Immunol 10, 2143.
- Schwartz C and Voehringer D (2020) Methods Mol Biol 2163, 367-375.
- Wada T et al. (2010) J Clin Invest 120, 2867-75.
- El Hachem C et al. (2018) Eur J Immunol 48, 861-873.
Alternate Names
BB139338; Mast cell protease 8; MCPT8; MMCP-; mMCP-8
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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