mAID-Tag (F4L5V) Rabbit Monoclonal Antibody #28723

Auxins are a family of plant hormones necessary for regulation of growth and development (1). In Arabidopsis thaliana, indole-3-acetic acid (IAA) is the most prominent auxin, and functions to target transcription factors containing a conserved auxin-inducible degron (AID) sequence to the Skp/Cullin/F-box (SCF) E3 ubiquitin ligase complex for degradation (1,2). By recombining the AID tag sequence into eukaryotic cells that lack the auxin response, rapid and reversible targeted protein degradation can be engineered by taking advantage of the host SCF E3 ligase complex (3). AID-tagged proteins can functionally replace their untagged counterparts without regard for protein localization (4), and the inducible degradation, via exogenous auxins or synthetic auxin-like compounds, allows for temporary depletion of proteins that are otherwise critical to cell viability (5).

The AID system has been adapted and improved since its introduction. The minimum sequence of auxin-responsive protein IAA17 necessary for the degron response was identified within amino acids Lys65-Lys132, dubbed mini-AID (mAID), allowing for increasingly smaller insertion sequences over time (6,7). More recently, the AID2 modifications improved the efficiency of the mAID system with the co-expression of a modified Oryza sativa E3 ligase TIR1 (OsTIR1), along with the use of a high-potency ligand 5-phenyl-indole-acetic acid (5-Ph-IAA) (8,9). Precise control of OsTIR1 expression during the cell cycle allows for phase-specific deletion of proteins of interest (10). Researchers have utilized CRISPR/Cas9-mediated homology-directed repair (HDR) to introduce homozygous knock-ins of mAID-tags to proteins of interest (11).