The LAPosome Antibody Sampler Kit provides an economical means of analyzing the proteins in the LAPosome complex. 该试剂盒包含的抗体足以使用每种一抗进行两次蛋白印迹实验。
Each antibody in the LAPosome Antibody Sampler Kit detects endogenous levels of its target protein. Rubicon (D9F7) Rabbit mAb 可检测在 55 kDa 处的来源不明的条带。
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Thr72 of human Beclin-1, Leu210 of human Rubicon, Lys630 of PI3 kinase class III, and Gly502 of human UVRAG. 使用与人 PIK3R4 蛋白中 Gly825 周围的残基相对应的合成肽对动物进行免疫接种来产生多克隆抗体。使用蛋白质 A 和肽亲和色谱对抗体进行纯化。
LC3-associated phagocytosis (LAP) is a process at the crossroads between autophagy and phagocytosis in which uptake of extracellular particles into phagophores utilizes components of the autophagic machinery and is targeted to the lysosome for degradation (reviewed in 1-3). Activation of LAP by infectious agents through engagement of pathogen receptors like Toll-like receptors (TLRs) are important in immune regulation and host defense. While autophagosomes and LAP phagophores both express lipidated LC3, autophagosomes are double membrane vesicles while the phagophores in LAP are single membrane. While LAP shares some components of canonical autophagy, there are some distinct differences. Both autophagy and LAP require PI3 Kinase Class III (PI3KC3; also known as VPS34) as well as the regulatory protein PIK3R4 (also known as VPS15) and Beclin-1. However, while canonical autophagy requires Atg14 and Ambra-1 in this complex, LAP requires Rubicon and UVRAG. This complex, known as the LAPosome, activates PI3KC3 and triggers nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (NOX2), generating reactive oxygen species (ROS) for phagophore maturation. The expression of Rubicon in this complex has emerged as a defining characteristic of LAP.