Name: LAMC2 (E9F7M) Rabbit Monoclonal Antibody
Price: 376.00 USD
Availability: InStock

Filter:

WB
IF

Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	105, 150
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunofluorescence (Immunocytochemistry)	1:1000 - 1:2000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

LAMC2 (E9F7M) Rabbit Monoclonal Antibody recognizes endogenous levels of total LAMC2 protein. This antibody detects both full-length (150 kDa) and processed (105 kDa) LAMC2 proteins.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His747 of human LAMC2 protein.

Background

Laminins are a family of proteins important for maintaining cellular basement membrane (BM) structure and function (1). Laminins exist as heterotrimers of α (LAMA), β (LAMB), and γ (LAMC) subunits. LAMC2 is the γ subunit of laminin-5 (LN-332 or LN5), which is a triplex composed of laminin α3, β3, and γ2 chains. LN-332 is one of the main isoforms of laminin associated with malignant transformation, proliferation, adhesion, migration, invasion, and metastasis (2). Upregulation of LAMC2 is a prognostic marker for the progression of various cancer types, including colorectal carcinoma, urinary bladder carcinoma, pancreatic ductal adenocarcinoma, and lung carcinoma (3-6). Under physiological conditions, LN-332 locates at the dermal-epidermal junction, provides skin integrity and resistance to external mechanical forces, and promotes adhesion and migration of epithelial cells (7,8). Mutation or deletion of the LAMC2 gene causes Herlitz junctional epidermolysis bullosa, an inherited disease causing severe skin blistering (9,10). The biological activity of LN-332 can be regulated by LAMC2 proteolytic processing through MT1-MMP (MMP14) (11). LN-332 containing full-length LAMC2 (150 kDa) functions to promote adhesion, while LN-332 containing processed LAMC2 (105 kDa) promotes migration (12).

Alternate Names

B2T; BM600; BM600-100kDa; Cell-scattering factor 140 kDa subunit; CSF; CSF 140 kDa subunit; EBR2; EBR2A; Epiligrin subunit gamma; kalinin (105kD); Kalinin subunit gamma; kalinin-105kDa; Kalinin/nicein/epiligrin 100 kDa subunit; ladsin (140kDa); Ladsin 140 kDa subunit; LAMB2T; LAMC2; Laminin B2t chain; laminin subunit gamma 2; Laminin subunit gamma-2; Laminin-5 subunit gamma; laminin, gamma 2; LAMNB2; Large adhesive scatter factor 140 kDa subunit; MGC138491; MGC141938; nicein (100kDa); Nicein subunit gamma; nicein-100kDa

Database Links

UniProt ID: Q13753

Entrez-Gene Id: 3918

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明，且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的，或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的，或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

For Research Use Only. Not for Use in Diagnostic Procedures.

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LAMC2 (E9F7M) Rabbit Monoclonal Antibody #53884