Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
JAK2 (V617F Mutant Specific) (F3W4N) Rabbit Monoclonal Antibody #66939
Filter:
- WB
Product Specifications
| REACTIVITY | H |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 125 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
实验步骤
Specificity / Sensitivity
JAK2 (V617F Mutant Specific) (F3W4N) Rabbit Monoclonal Antibody recognizes transfected levels of JAK2 V617F protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to V617F mutant sequence of human JAK2 protein.
Background
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
Approximately 70-75% of myeloproliferative neoplasms (MPNs) are driven by the V617F mutation in JAK2 (6). The discovery of JAK2 V617F has helped elucidate the pathogenic mechanism of MPNs, and the mutation has prognostic impact and potential as a therapeutic target (7).
Approximately 70-75% of myeloproliferative neoplasms (MPNs) are driven by the V617F mutation in JAK2 (6). The discovery of JAK2 V617F has helped elucidate the pathogenic mechanism of MPNs, and the mutation has prognostic impact and potential as a therapeutic target (7).
- Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
- Darnell, J.E. (1997) Science 277, 1630-5.
- VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
- Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
- Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
- Vainchenker, W. et al. (2011) Blood 118, 1723-35.
- Zhang, Y. et al. (2024) Cancer 130, 4229-4240.
Alternate Names
JAK-2; JAK2; Janus kinase 2; Janus kinase 2 (a protein tyrosine kinase); JTK10; Tyrosine-protein kinase JAK2
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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