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ILF3 (E5H6F) Rabbit Monoclonal Antibody

ILF3 (E5H6F) Rabbit Monoclonal Antibody #39249

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Product Specifications

REACTIVITY	H Mk
SENSITIVITY	Endogenous
MW (kDa)	85, 110
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry
IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:1000 - 1:4000
Immunofluorescence (Immunocytochemistry)	1:200 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

ILF3 (E5H6F) Rabbit Monoclonal Antibody recognizes endogenous levels of total ILF3 protein. Species reactivity for IHC-P is human only.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val353 of human ILF3 protein.

Background

ILF2 and ILF3, also known as NF45 and NF90 respectively, form a double-stranded RNA binding heterodimer that serves many different biological functions. ILF2 and ILF3 were originally discovered as proteins that can bind and regulate the antigen receptor response element of the IL2 promoter to contribute to T cell activation (1,2). ILF2 and ILF3 play important roles in DNA damage response and non-homologous end joining (NHEJ) by interacting with DNA-PK (3,4). The heterodimer can also bind and negatively regulate microRNAs (5,6). Biogenesis of the 60S ribosomal subunit has been shown to be dependent on the double-stranded RNA-binding domains of ILF3 (7). Additionally, ILF2 and ILF3 can mediate rDNA transcription (8). ILF2 and ILF3 also play a role in the life cycle of many different viruses, including dengue and hepatitis C (9-11).

Alternate Names

CBTF; Double-stranded RNA-binding protein 76; double-stranded RNA-binding protein, 76 kD; DRBF; DRBP76; dsRNA binding protein NFAR-2/MPP4; ILF3; interleukin enhancer binding factor 3; interleukin enhancer binding factor 3, 90kD; interleukin enhancer binding factor 3, 90kDa; Interleukin enhancer-binding factor 3; M phase phosphoprotein 4, nuclear factor associated with DS RNA; M-phase phosphoprotein 4; MMP4; MPHOSPH4; MPP4; MPP4110; NF-AT-90; NF110; NF110b; NF90; NF90a; NF90b; NF90c; NF90ctv; NFAR; NFAR-1; NFAR-2; NFAR110; NFAR2; NFAR90; NFAT90; Nuclear factor associated with dsRNA; nuclear factor of activated T-cells 110 kDa; Nuclear factor of activated T-cells 90 kDa; nuclear factor of activated T-cells, 90 kD; TCP110; TCP80; Translational control protein 80

Database Links

UniProt ID: Q12906

Entrez-Gene Id: 3609

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

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For Research Use Only. Not for Use in Diagnostic Procedures.

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