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  3. HSP60 (D307) Antibody

HSP60 (D307) Antibody #4870

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    Product Specifications

    REACTIVITY H M R Mk Dm
    SENSITIVITY Endogenous
    MW (kDa) 60
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Dm-D. melanogaster 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:50
    Flow Cytometry (Fixed/Permeabilized) 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    实验步骤

    Specificity / Sensitivity

    HSP60 (D307) Antibody detects endogenous levels of total HSP60 protein. This antibody does not cross-react with other HSPs.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, D. melanogaster

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Asp307 of human HSP60. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    In both prokaryotic and eukaryotic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). Research studies have shown that a significant amount of HSP60 is also present in the cytosol of many cells, and that it is induced by stress, inflammatory and immune responses, and autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8).

    Alternate Names

    60 kDa chaperonin; 60 kDa heat shock protein, mitochondrial; CH60; Chaperonin 60; CPN60; epididymis secretory sperm binding protein; GROEL; heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 65; heat shock protein family D (Hsp60) member 1; HLD4; HSP-60; HSP60; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; SPG13

    Database Links

    UniProt ID: P10809


    Entrez-Gene Id: 3329


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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