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HSD3B1 (F7Q2L) Rabbit Monoclonal Antibody

HSD3B1 (F7Q2L) Rabbit Monoclonal Antibody #15230

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Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	42
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

HSD3B1 (F7Q2L) Rabbit Monoclonal Antibody recognizes endogenous levels of total HSD3B1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro154 of human HSD3B1 protein.

Background

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (HSD3B1), also known as 3β-hydroxysteroid dehydrogenase-1 (3βHSD1), is critical for steroid hormone biosynthesis (1,2). This enzyme catalyzes the rate-limiting step of extragonadal androgen synthesis. Studies show that glucosamine released from cancer-associated fibroblasts (CAFs) enhances O-GlcNAcylation in prostate cancer cells, activating HSD3B1/3βHSD1 expression mediated by the transcription factor Elk1. This increases de novo intratumoral androgen biosynthesis and leads to castration-resistant prostate cancer (CRPC) (2). In addition, the adrenal-permissive HSD3B1 (1245C) allele, which upregulates intratumoral androgen biosynthesis, augments resistance to radiotherapy in prostate cancer (3).

Alternate Names

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-hydroxysteroid 3-dehydrogenase; 3BETAHSD; 3BH; 3BHS1; Delta-5-3-ketosteroid isomerase; Dihydrotestosterone oxidoreductase; HSD3B; HSD3B1; HSDB3; HSDB3A; hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1; hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1; I; Progesterone reductase; SDR11E1; short chain dehydrogenase/reductase family 11E, member 1; Steroid Delta-isomerase; Trophoblast antigen FDO161G

Database Links

UniProt ID: P14060

Entrez-Gene Id: 3283

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

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For Research Use Only. Not for Use in Diagnostic Procedures.

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