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  3. HCAR1/GPR81 (F9I8Q) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

HCAR1/GPR81 (F9I8Q) Rabbit Monoclonal Antibody #20689

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 30-60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:50 - 1:200
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    实验步骤

    Specificity / Sensitivity

    HCAR1/GPR81 (F9I8Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total HCAR1 protein. This product shows nonspecific staining in stomach crypts by immunohistochemistry.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human HCAR1 protein.

    Background

    The hydroxycarboxylic acid receptor 1 (HCAR1), formerly known as GPR81, is a G protein-coupled receptor (GPCR) for endogenous lactate, connecting energy metabolism with neural function (1). HCAR1 was initially described in adipocytes, where its activation inhibits of lipolysis via the Gi-dependent intracellular pathway activation (1,2). More recently, HCAR1 has been identified to modulate neuronal network activity through the activation of Gα and Gβγ subunits (3). Furthermore, HCAR1 was found to be a key modulator of pathways that promote tissue regeneration after hypoxia-ischemia and mitigate epileptic seizure activity in the brain (4,5). Besides its role in neurons, HCAR1 also directly influences astrocyte development and plays a role in metabolic regulation in glial cells during hypoglycemia (6).

    Alternate Names

    FKSG80; G protein-coupled receptor 104; G protein-coupled receptor 81; G-protein coupled receptor 104; G-protein coupled receptor 81; GPR104; GPR81; HCA1; HCAR1; hydroxy-carboxylic acid receptor 1; Hydroxycarboxylic acid receptor 1; LACR1; lactate receptor 1; T-cell activation G protein-coupled receptor; TA-GPCR; TAGPCR

    Database Links

    UniProt ID: Q9BXC0


    Entrez-Gene Id: 27198


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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