Name: GPAT3 (E5R1H) Rabbit Monoclonal Antibody
Price: 351.00 USD
Availability: InStock

Filter:

WB
IP

Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	40
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

GPAT3 (E5R1H) Rabbit Monoclonal Antibody recognizes endogenous levels of total GPAT3 protein. Background bands of unknown identity are detected at 20, 70, and 170 kDa.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His289 of human GPAT3 protein.

Background

Glycerol-3-phosphate acyltransferases (GPATs) catalyze the first step in the de novo synthesis of triacylglycerol (TAG) and phospholipids by converting glycerol-3-phosphate to lysophosphatidic acid (LPA) (1). The GPAT family consists of four family members: two mitochondrial proteins (GPAT1 and GPAT2) and two microsomal proteins (GPAT3 and GPAT4). Each can differ in tissue expression, regulation, and physiological and pathophysiological roles (2). The microsomal proteins GPAT3 and GPAT4 were originally designated as AGPAT8 and AGPAT6, respectively. 1-acyl-glycerol-3-phosphate acyltransferase (AGPAT) catalyzes the subsequent reaction converting LPA to phosphatidic acid. The genes were renamed to GPAT3 and GPAT4 upon elucidation of GPAT activity (3,4). Both GPAT3 and GPAT4 are expressed in adipose tissue, but GPAT3 is highly induced during adipocyte differentiation (4). Brown adipose tissue (BAT) preferentially expresses GPAT4 (5). GPAT3 and GPAT4 may also be phosphorylated in response to insulin treatment to increase GPAT activity (6). Accumulation of saturated LPA produced by GPAT4 at the ER-mitochondrial contact site can inhibit autophagy through the abnormal formation of omegasomes, which are precursors to the autophagosome (7). Glycerolipid synthesis by GPAT3 and GPAT4 can also regulate activation of macrophages (8).

Alternate Names

1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10; 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9; 1-acylglycerol-3-phosphate O-acyltransferase 8; 1-acylglycerol-3-phosphate O-acyltransferase 9; 1-AGP acyltransferase 9; 1-AGPAT 9; Acyl-CoA:glycerol-3-phosphate acyltransferase 3; AGPAT 10; AGPAT10; AGPAT8; AGPAT9; endoplasmic reticulum associated GPAT; Glycerol-3-phosphate acyltransferase 3; GPAT-3; GPAT3; hGPAT3; HMFN0839; LPAAT-theta; Lung cancer metastasis-associated protein 1; Lysophosphatidic acid acyltransferase theta; lysophosphatidic acid acyltransferase, theta; MAG-1; MAG1; MGC11324; testis secretory sperm-binding protein Li 213e

Database Links

UniProt ID: Q53EU6

Entrez-Gene Id: 84803

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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GPAT3 (E5R1H) Rabbit Monoclonal Antibody #71236