Name: GAD1 (D1F2M) Horse Chimeric Monoclonal Antibody
Price: 160.00 USD
Availability: InStock

Filter:

IF

Product Specifications

REACTIVITY	M R
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Horse chimeric IgG4

Application Key:

IF-Immunofluorescence

Species Cross-Reactivity Key:

M-Mouse
R-Rat

Product Information

Product Description

This Cell Signaling Technology^® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as GAD1 (D1F2M) Rabbit Monoclonal Antibody #41318.

Product Usage Information

Application	Dilution
Immunofluorescence (Frozen)	1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

GAD1 (D1F2M) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of total GAD1 protein.

Species Reactivity:

Mouse, Rat

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Human

Source / Purification

This recombinant chimeric antibody is engineered from GAD1 (D1F2M) Rabbit Monoclonal Antibody #41318 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

The parent antibody, GAD1 (D1F2M) Rabbit Monoclonal Antibody #41318, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala15 of human GAD1protein.

Background

The enzyme glutamate decarboxylase (GAD) is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid (1). GAD1 (GAD67) and GAD2 (GAD65) are expressed in nervous and endocrine systems (2) and are thought to be involved in synaptic transmission (3) and insulin secretion (4), respectively. Autoantibodies against GAD2 may serve as markers for type I diabetes (5). Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2 (6).
GAD1 and GAD2 are encoded by separate genes and are coexpressed in most of the GABA-containing neurons (1, 7).

Alternate Names

67 kDa glutamic acid decarboxylase; CPSQ1; DCE1; FLJ45882; GAD; GAD-67; GAD1; GAD67; Glutamate decarboxylase 1; glutamate decarboxylase 1 (brain, 67kDa); Glutamate decarboxylase 67 kDa isoform; SCP

Database Links

UniProt ID: Q99259

Entrez-Gene Id: 2571

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

专品专有“专供研究使用”的专专或专似的专专声明，且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的，或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的，或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

For Research Use Only. Not for Use in Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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GAD1 (D1F2M) Horse Chimeric Monoclonal Antibody #24655