Name: ETV4 (E5Q1U) Rabbit Monoclonal Antibody
Price: 371.00 USD
Availability: InStock

Filter:

WB
IP
IF

Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	54-61
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:200
Immunofluorescence (Immunocytochemistry)	1:800 - 1:3200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ETV4 (E5Q1U) Rabbit Monoclonal Antibody recognizes endogenous levels of total ETV4 protein. This antibody does not cross-react with ETV5 protein. Based on homology and observed expression, this antibody is not predicted to cross-react with ETV1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human ETV4 protein. The antigen has been further categorized as corresponding to residues surrounding Gly144 of human ETV4 protein.

Background

ETS translocation variant 4 (ETV4, PEA3, E1AF) is a member of the PEA3 (polyomavirus enhancer activator 3) subfamily of the E26 transformation-specific (ETS) DNA-binding transcription factors, which commonly bind a core DNA sequence of 5'-GGA(A/T)-3' (1,2). The PEA3 subfamily of ETV1, ETV4, and ETV5 are considered oncogenic transcription factors due to their overexpression and promotion of tumorigenesis in numerous cancers (2,3). ETV4 can be activated via the RAS-RAF-MEK-ERK pathway (2,4) or the PI3K/Akt pathway (5), and loss-of-function mutations of PEA3-family repressors such as CIC can increase ETV4 expression independent of either pathway (2,6). Though rare, 5' chromosomal fusions to ETV4 are drivers of metastasis in prostate cancer (7) and Ewing sarcoma (8). ETV4 regulates the expression of genes that control several developmental processes, including cell growth, differentiation, proliferation, survival, and cell cycle progression (9-11).

Alternate Names

Adenovirus E1A enhancer-binding protein; E1A enhancer binding protein; E1A-F; E1AF; ETS translocation variant 4; ets variant 4; ets variant gene 4 (E1A enhancer binding protein, E1AF); ets variant gene 4 (E1A enhancer-binding protein, E1AF); ETS variant transcription factor 4; ETV4; EWS protein/E1A enhancer binding protein chimera; PEA3; PEAS3; Polyomavirus enhancer activator 3 homolog; polyomavirus enhancer activator-3; Protein PEA3

Database Links

UniProt ID: P43268

Entrez-Gene Id: 2118

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

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For Research Use Only. Not for Use in Diagnostic Procedures.

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ETV4 (E5Q1U) Rabbit Monoclonal Antibody #19200