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  3. ERCC1 (D61F5) Rabbit Monoclonal Antibody
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ERCC1 (D61F5) Rabbit Monoclonal Antibody #5437

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 39
    Source/Isotype Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    实验步骤

    Specificity / Sensitivity

    ERCC1 (D61F5) Rabbit Monoclonal Antibody recognizes endogenous levels of total ERCC1 isoforms 1, 2 and 3 protein. Experiments utilizing overexpression constructs indicated that this antibody does not cross-react with ERCC1 isoform 4.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ERCC1 protein.

    Background

    DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals (1). Excision Repair Cross Complementing 1 (ERCC1) forms a complex with ERCC4/XPF, which acts as the 5’ endonuclease required to excise the lesion (2). ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) (3) and involved in repair of double strand breaks (4). Research studies have shown that expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (NSCLC) (5,6).

    Alternate Names

    COFS4; DNA excision repair protein ERCC-1; ERCC excision repair 1, endonuclease non-catalytic subunit; ERCC1; excision repair cross-complementation group 1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence); RAD10; UV20

    Database Links

    UniProt ID: P07992


    Entrez-Gene Id: 2067


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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