EPRS Antibody #45956
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 180 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
EPRS Antibody recognizes endogenous levels of total EPRS protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro978 of human EPRS protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
EPRS (Glutamatyl-prolyl-tRNA synthetase) is a bifunctional enzyme in the aminoacyl-tRNA ligase family that attaches the cognate amino acid to the corresponding tRNA for protein translation (1,2). EPRS usually resides in the tRNA multisynthetase complex (MSC) that may facilitate the delivery of aminoacylated tRNAs to the ribosome during protein synthesis (3,4). In monocytic cells, upon interferon (IFN)-gamma activation, EPRS becomes phosphorylated and is released from the MSC to form the so-called GAIT (IFN-Gamma-Activated Inhibitor of Translation) complex with NS1-associated protein (NSAP1), ribosomal protein L13a, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The GAIT complex binds to a defined RNA element through EPRS in the 3’ untranslated region (UTR) to inhibit translation of target transcripts, including vascular endothelial growth factor (VEGF)-A, ceruloplasmin, and several cytokines and their receptors. Thus, EPRS plays an important role in inflammation regulation (5-9).
- Ibba, M. and Soll, D. (2000) Annu Rev Biochem 69, 617-50.
- Ribas de Pouplana, L. and Schimmel, P. (2001) Trends Biochem Sci 26, 591-6.
- Kyriacou, S.V. and Deutscher, M.P. (2008) Mol Cell 29, 419-27.
- Hausmann, C.D. and Ibba, M. (2008) FEMS Microbiol Rev 32, 705-21.
- Sampath, P. et al. (2004) Cell 119, 195-208.
- Sampath, P. et al. (2003) Mol Cell Biol 23, 1509-19.
- Ray, P.S. and Fox, P.L. (2007) EMBO J 26, 3360-72.
- Vyas, K. et al. (2009) Mol Cell Biol 29, 458-70.
- Arif, A. et al. (2018) Wiley Interdiscip Rev RNA 9, .
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