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  3. DHX9 (E6M4Q) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DHX9 (E6M4Q) Rabbit Monoclonal Antibody #71286

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  • WB
  • ChIP

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 145
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Chromatin IP 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    实验步骤

    Specificity / Sensitivity

    DHX9 (E6M4Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total DHX9 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile23 of human DHX9 protein.

    Background

    DExH-box helicase 9 (DHX9), also known as nuclear DNA helicase II or RNA helicase A, is a protein involved in unwinding DNA and RNA by using NTP (1). DHX9 can translocate in the 3’ to 5’ direction and can utilize all dNTPs or rNTPs, and contributes to pre-mRNA splicing (2,3). Essential for embryo development, DHX9 plays roles in many biological processes, including DNA replication, self-renewal, dosage compensation, and neuronal development (4-7). DHX9 is a critical regulator of abnormal DNA secondary structures (8). It is recruited by TDRD3 to R-loops, where it can unwind and resolve them to prevent DNA damage (9-12). DHX9 plays a central role in the formation and resolution of R-loops (8-11). It also can bind to G-quadruplexes and inverted-repeat Alu elements, suppressing formation of circular RNAs (9,13). BRCA1 is recruited to RNA by DHX9 to promote homologous recombination (14). Furthermore, DHX9 has been shown to be upregulated in various cancer types and can also play a role in viral replication (15-17).
    1. Zhang, S. and Grosse, F. (1994) Biochemistry 33, 3906-12.
    2. Lee, C.G. and Hurwitz, J. (1992) J Biol Chem 267, 4398-407.
    3. Zhang, S. et al. (1999) J Cell Sci 112 (Pt 7), 1055-64.
    4. Friedemann, J. et al. (2005) J Biol Chem 280, 31303-13.
    5. Närvä, E. et al. (2012) Stem Cells 30, 452-60.
    6. Kuroda, M.I. et al. (1991) Cell 66, 935-47.
    7. Hoekstra, E.J. et al. (2013) PLoS One 8, e53122.
    8. Jain, A. et al. (2013) Nucleic Acids Res 41, 10345-57.
    9. Chakraborty, P. and Grosse, F. (2011) DNA Repair (Amst) 10, 654-65.
    10. Chakraborty, P. et al. (2018) Nat Commun 9, 4346.
    11. Cristini, A. et al. (2018) Cell Rep 23, 1891-1905.
    12. Yuan, W. et al. (2021) Nucleic Acids Res 49, 8573-8591.
    13. Aktaş, T. et al. (2017) Nature 544, 115-119.
    14. Chakraborty, P. and Hiom, K. (2021) Nat Commun 12, 4126.
    15. Shi, F. et al. (2021) J Clin Lab Anal 35, e24052.
    16. Xiao, L. et al. (2022) J Cancer 13, 102-111.
    17. Guo, F. and Xing, L. (2021) Virus Res 291, 198206.

    Alternate Names

    ATP-dependent RNA helicase A; DDX9; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9; DEAH (Asp-Glu-Ala-His) box helicase 9; DEAH (Asp-Glu-Ala-His) box polypeptide 9; DEAH box protein 9; DEAH-box helicase 9; DExH-box helicase 9; DHX9; leukophysin; LKP; NDH II; NDH2; NDHII; Nuclear DNA helicase II; RHA; RNA helicase A

    Database Links

    UniProt ID: Q08211


    Entrez-Gene Id: 1660


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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