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  3. DDX42 (F4V2L) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DDX42 (F4V2L) Rabbit Monoclonal Antibody #90370

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    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 130
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    实验步骤

    Specificity / Sensitivity

    DDX42 (F4V2L) Rabbit Monoclonal Antibody recognizes endogenous levels of total DDX42 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly63 of human DDX42 protein.

    Background

    DEAD box protein 42 (DDX42) is an ATP-dependent DEAD box RNA helicase that has key functions in splicing. DDX42 has RNA chaperone activity and associates with the SF3b complex along with DDX46, facilitating 17S U2 snRNP assembly (1-4). In oncogenic SF3B1 V700E mutations, interactions between SF3B1 and DDX42/46 are greatly reduced, resulting in alternative splicing (5). DDX42 also plays a key role in the inhibition of positive-strand RNA viruses, including HIV and SARS-CoV-2 (6,7). In addition to its role in splicing, DDX42 is capable of binding G-quadruplexes, higher-order structures in the genome which can be aberrantly accumulated in cancer (8).

    Alternate Names

    ATP-dependent RNA helicase DDX42; DDX42; DDX42P; DEAD (Asp-Glu-Ala-Asp) box helicase 42; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DEAD box protein 42; DEAD-box helicase 42; FLJ43179; RHELP; RNA helicase-like protein; RNA helicase-related protein; RNAHP; SF3b DEAD box protein; SF3b125; SF3b125 DEAD-box protein; SF3B8; Splicing factor 3B-associated 125 kDa protein; splicing factor 3b, subunit 8

    Database Links

    UniProt ID: Q86XP3


    Entrez-Gene Id: 11325


    限制使用

    除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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