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  3. Dab2 (F9K9T) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Dab2 (F9K9T) Rabbit Monoclonal Antibody #27376

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  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 96
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    实验步骤

    Specificity / Sensitivity

    Dab2 (F9K9T) Rabbit Monoclonal Antibody recognizes endogenous levels of total Dab2 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly547 of human Dab2 protein.

    Background

    Disabled homolog 2 (Dab2) is a mitogen responsive phosphoprotein that exerts multiple functions through association with numerous proteins. Dab2 modulates signaling pathways through interactions with proteins such as Smads and TGF-β receptors (1,2), axin (3), GRB (4), and Src (5). Dab2 also serves as a cargo-specific adaptor of clathrin-mediated endocytosis via interaction with clathrin (6), AP2 (7), NPXY-containing cargo (8-10), and myosin VI (11,12). In addition, Dab2 regulates cell adhesion by directly binding integrins (13,14). The diverse functions of Dab2 enable it to coordinate cell adhesion, cell motility, membrane trafficking, and signaling. Research studies have shown Dab2 is downregulated in a number of cancers, thereby suggesting a role as a tumor suppressor (15-17). Phosphorylation of Dab2 decreases its endocytotic function (18).
    1. Hocevar, B.A. et al. (2001) EMBO J 20, 2789-801.
    2. Hocevar, B.A. et al. (2005) J Biol Chem 280, 25920-7.
    3. Hocevar, B.A. et al. (2003) EMBO J 22, 3084-94.
    4. Xu, X.X. et al. (1998) Oncogene 16, 1561-9.
    5. Zhou, J. et al. (2003) J Biol Chem 278, 6936-41.
    6. Mishra, S.K. et al. (2002) EMBO J 21, 4915-26.
    7. Morris, S.M. and Cooper, J.A. (2001) Traffic 2, 111-23.
    8. Keyel, P.A. et al. (2006) Mol Biol Cell 17, 4300-17.
    9. Maurer, M.E. and Cooper, J.A. (2006) J Cell Sci 119, 4235-46.
    10. Maurer, M.E. and Cooper, J.A. (2005) J Cell Sci 118, 5345-55.
    11. Morris, S.M. et al. (2002) Traffic 3, 331-41.
    12. Hasson, T. (2003) J Cell Sci 116, 3453-61.
    13. Huang, C.L. et al. (2006) J Cell Sci 119, 4420-30.
    14. Teckchandani, A. et al. (2009) J Cell Biol 186, 99-111.
    15. Mok, S.C. et al. (1998) Oncogene 16, 2381-7.
    16. Anupam, K. et al. (2006) World J Gastroenterol 12, 6041-5.
    17. Bagadi, S.A. et al. (2007) Breast Cancer Res Treat 104, 277-86.
    18. Chetrit, D. et al. (2011) J Biol Chem 286, 5392-403.

    Alternate Names

    Adaptor molecule disabled-2; DAB adaptor protein 2; Dab, mitogen-responsive phosphoprotein, homolog 2; DAB2; DAB2, clathrin adaptor protein; Differentially expressed in ovarian carcinoma 2; Differentially-expressed protein 2; Disabled homolog 2; disabled homolog 2, mitogen-responsive phosphoprotein; disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila); DOC-2; DOC2; FLJ26626; mitogen-responsive phosphoprotein

    Database Links

    UniProt ID: P98082


    Entrez-Gene Id: 1601


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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