Name: COL17A1 (F5U1Z) Rabbit Monoclonal Antibody
Price: 178.00 USD
Availability: InStock

Filter:

WB
IHC

Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	190
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:400 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

COL17A1 (F5U1Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total COL17A1 protein. This antibody detects a 62 kDa band, likely a processed N-terminal fragment of COL17A1. Non-specific staining was observed in some peripheral nerves, kidney proximal tubules, and small intestine epithelium by immunohistochemistry.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr102 of human COL17A1 protein.

Background

Collagen type XVII alpha 1 chain (COL17A1/BP180) is a collagen family hemidesmosome anchorage molecule of basal keratinocytes that promotes stable epidermal-dermal adhesion and wound healing (1). COL17A1 is critical for maintaining keratocyte and hair follicle stem cells, promoting cell motility and increased self-renewing capability (2-4). The protein functions by organizing actin and keratin filament networks via its interaction with intracellular scaffold protein plectin (PLEC) and integrin β4 in the hemidesmosomes (2,5). High expression of COL17A1 is associated with cervical, colorectal, pancreatic, and lung cancers (6-9). It induces a tumor-favorable microenvironment and promotes invasion and metastasis. Conversely, decreased expression of COL17A1 in breast cancer is associated with increased cancer migration and invasion (10). COL17A1 was found to promote cancer dormancy and chemoresistance in colorectal stem cells (11,12). COL17A1 mutations and autoantibodies against COL17A1 can cause blistering skin diseases due to loss of attachment of the epidermis to the underlying basement membrane (13,14).

Alternate Names

120 kDa linear IgA dermatosis antigen; 120 kDa linear IgA disease antigen; 180 kDa bullous pemphigoid antigen 2; 97 kDa LAD antigen; 97 kDa linear IgA bullous dermatosis antigen; 97 kDa linear IgA disease antigen; 97-LAD; alpha 1 type XVII collagen; BA16H23.2; bA16H23.2 (collagen, type XVII, alpha 1 (BP180)); BP180; BPA-2; BPAG2; Bullous pemphigoid antigen 2; bullous pemphigoid antigen 2 (180kD); COHA1; COL17A1; Collagen alpha-1(XVII) chain; collagen type XVII alpha 1 chain; collagen XVII, alpha-1 polypeptide; collagen, type XVII, alpha 1; ERED; FLJ60881; KIAA0204; LABD97; LAD-1; Linear IgA bullous disease antigen of 97 kDa; Linear IgA disease antigen 1; type XVII collagen alpha-1

Database Links

UniProt ID: Q9UMD9

Entrez-Gene Id: 1308

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

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For Research Use Only. Not for Use in Diagnostic Procedures.

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COL17A1 (F5U1Z) Rabbit Monoclonal Antibody #67360