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  3. CHD8 Antibody

CHD8 Antibody #7656

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 290
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    实验步骤

    Specificity / Sensitivity

    CHD8 Antibody recognizes endogenous levels of total CHD8 protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CHD8 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    CHD8 belongs to the chromodomain helicase DNA-binding (CHD) family of ATP-dependent chromatin remodeling proteins (1). The CHD family of proteins has been shown to play an important role in regulating gene expression by utilizing the energy derived from ATP hydrolysis to alter chromatin architecture (1,2). The nine CHD family members are characterized by the presence of two tandem chromodomains in the N-terminal region and an SNF2-like ATPase domain near the central region of the protein (2-4). In addition, CHD8 contains three CR (conserved region) domains, a SANT (switching-defective protein 3, adaptor 2, nuclear receptor co-repressor, transcription factor IIB)-like domain, two BRK (brahma and kismet) domains, and a DNA-binding domain (2). The chromatin remodeling activity of CHD8 has been shown to be important for the regulation of a wide variety of genes, such as the HOX genes (5) and genes that are driven by β-catenin (6), p53 (7), estrogen receptor (8), or androgen receptor (9). CHD8 can also interact with the insulator binding protein CTCF and is required for CTCF insulator activity at multiple gene loci (10).

    Alternate Names

    ATP-dependent helicase CHD8; AUTS18; axis duplication inhibitor; beta-catenin binding protein-like; CHD-8; CHD8; chromodomain helicase DNA binding protein 8; Chromodomain-helicase-DNA-binding protein 8; DKFZp686N17164; duplin; Helicase with SNF2 domain 1; HELSNF1; KIAA1564

    Database Links

    UniProt ID: Q9HCK8


    Entrez-Gene Id: 57680


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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