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CD31 (PECAM-1) (D8V9E) Feline Chimeric Monoclonal Antibody

CD31 (PECAM-1) (D8V9E) Feline Chimeric Monoclonal Antibody #52160

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Product Specifications

REACTIVITY	M
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Feline chimeric IgG1

Application Key:

IHC-Immunohistochemistry

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Description

This Cell Signaling Technology^® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as CD31 (PECAM-1) (D8V9E) Rabbit Monoclonal Antibody #77699.

Product Usage Information

Application	Dilution
Immunohistochemistry (Paraffin)	1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

CD31 (PECAM-1) (D8V9E) Feline Chimeric Monoclonal Antibody recognizes endogenous levels of total CD31 (PECAM-1) protein.

Species Reactivity:

Mouse

Source / Purification

This recombinant chimeric antibody is engineered from CD31 (PECAM-1) (D8V9E) Rabbit Monoclonal Antibody #77699 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

The parent antibody, CD31 (PECAM-1) (D8V9E) Rabbit Monoclonal Antibody #77699, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala451 of mouse CD31 (PECAM-1) protein.

Background

CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).

Alternate Names

adhesion molecule; CD31; CD31 antigen; CD31/EndoCAM; EndoCAM; FLJ58394; GPIIA'; PECA1; PECAM-1; PECAM-1, CD31/EndoCAM; PECAM1; platelet and endothelial cell adhesion molecule 1; Platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule

Database Links

UniProt ID: P16284

Entrez-Gene Id: 5175

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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