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Carbamidomethyl Cysteine (F3T7J) Rabbit Monoclonal Antibody #80353
Filter:
- WB
- F
Product Specifications
| REACTIVITY | All |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- F-Flow Cytometry
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
实验步骤
Specificity / Sensitivity
Carbamidomethyl Cysteine (F3T7J) Rabbit Monoclonal Antibody recognizes a broad range of carbamidomethylated cysteine containing proteins and peptides. This antibody does not cross-react with cysteines alkylated with desthiobiotin iodoacetamide (DBIA).
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing carbamidomethylated cysteine flanked by degenerate amino acids at positions N- and C-terminal to the modified cysteine.
Background
Cysteine alkylation is often performed with iodoacetamide (IAA) or chloroacetamide (CAA) to generate a carbamidomethyl (CAM) modification. Because alkylation occurs on reduced cysteines, disulfide-bonded cysteines must first be treated with dithiothreitol (DTT) or another reducing agent. The alkylation reaction is irreversible. Consequently, alkylated cysteines are unable to re-associate with each other. If the sulfhydryl group of a cysteine is already modified by another compound, that cysteine will not be able to react with IAA to form the carbamidomethyl modification. The alkylation reaction is rapid, although the efficiency can be influenced by the denaturant type used (1).
Proteins are often derivatized to prevent disulfide bond scrambling that can lead to artifactual bands in gel electrophoresis, and to minimize protease inaccessibility of cysteine-containing regions that can limit coverage in mass spectrometry-based proteomics. In light of various studies describing potential non-cysteine reactivities of IAA and CAA and re-evaluating the effectiveness of such reactions, users should consider the benefits of cysteine derivatizations depending on their specific experimental goals (2-4).
Proteins are often derivatized to prevent disulfide bond scrambling that can lead to artifactual bands in gel electrophoresis, and to minimize protease inaccessibility of cysteine-containing regions that can limit coverage in mass spectrometry-based proteomics. In light of various studies describing potential non-cysteine reactivities of IAA and CAA and re-evaluating the effectiveness of such reactions, users should consider the benefits of cysteine derivatizations depending on their specific experimental goals (2-4).
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not for Use in Diagnostic Procedures.
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