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BACE1 (D10E5) Mouse Chimeric Monoclonal Antibody

BACE1 (D10E5) Mouse Chimeric Monoclonal Antibody #17860

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Product Specifications

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Mouse chimeric IgG2a

Application Key:

IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Description

This Cell Signaling Technology^® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as BACE1 (D10E5) Rabbit Monoclonal Antibody #5606.

Product Usage Information

Application	Dilution
Immunofluorescence (Frozen)	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

BACE1 (D10E5) Mouse Chimeric Monoclonal Antibody detects endogenous levels of total BACE1 protein. This product does not detect BACE2.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

This recombinant chimeric antibody is engineered from BACE1 (D10E5) Rabbit Monoclonal Antibody #5606 according to animal-free protocols. This chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

The parent antibody, BACE1 (D10E5) Rabbit Monoclonal Antibody #5606, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His490 of human BACE1 protein.

Background

β-secretase (BACE) is an aspartic acid proteinase that catalyzes the initial step in APP processing by cleaving and releasing a soluble, extracellular APP-β (sAPPβ) ectodomain and generating a membrane-bound, carboxy-terminal fragment consisting of 99 amino acids (CTF99). Additional processing of CTF99 by γ-secretase generates the amyloid β-peptide (Aβ) that forms aggregates in the brains of Alzheimer disease (AD) patients. BACE is an attractive target for inhibitors in AD therapy since it catalyzes the first and rate limiting step in amyloidogenic APP processing (1). Pro-BACE-1 is synthesized in the ER before it is transported to the trans-Golgi network to undergo maturation (2). BACE-1 functions as a dimer and is phosphorylated at Ser498 by casein kinase 1, which is necessary for recycling back to the membrane from the early endosome (3,4).

Alternate Names

APP beta-secretase; Asp 2; ASP2; Aspartyl protease 2; BACE; BACE1; Beta-secretase 1; beta-secretase 1 precursor variant 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme; beta-site APP-cleaving enzyme 1; FLJ90568; HSPC104; KIAA1149; Memapsin-2; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2

Database Links

UniProt ID: P56817

Entrez-Gene Id: 23621

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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