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29483
Argonaute Antibody Sampler Kit
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Argonaute Antibody Sampler Kit #29483

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Western blot analysis of extracts from various cell types using Argonaute 2 (C34C6) Rabbit mAb.
Western blot analysis of extracts from various cell types using Argonaute 1 (D84G10) XP® Rabbit mAb.
Western blot analysis of extracts from PANC-1, HeLa, and C2C12 cells using Argonaute 3 (D15D2) Rabbit mAb.
Western blot analysis of extracts from various cell lines using Argonaute 4 (D10F10) Rabbit mAb.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Immunoprecipitation of Argonaute 2 from COS-7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Agonaute 2 (C34C6) Rabbit mAb. Western blot analysis was performed using Argonaute 2 (C34C6) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast using Argonaute 1 (D84G10) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human Argonaute 3 (hArgonaute 3-DDK; +), using Argonaute 3 (D15D2) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
To Purchase # 29483T
Cat. # Size Price Inventory
29483T
1 Kit  (4 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Argonaute 1 (D84G10) XP® Rabbit mAb 5053 20 µl
  • WB
  • IP
  • IHC
H M R Mk 97 Rabbit IgG
Argonaute 2 (C34C6) Rabbit mAb 2897 20 µl
  • WB
  • IP
H M R Mk 97 Rabbit IgG
Argonaute 3 (D15D2) Rabbit mAb 5054 20 µl
  • WB
  • IP
H M Mk 97 Rabbit IgG
Argonaute 4 (D10F10) Rabbit mAb 6913 20 µl
  • WB
  • IP
H M R Mk 97 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Argonaute Antibody Sampler Kit provides an economical means of evaluating total levels of Argonaute proteins. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Argonaute Antibody Sampler Kit detects endogenous levels of its target protein. Experiments utilizing overexpression constructs indicated that Argonaute 4 (D10F10) Rabbit mAb may weakly cross-react with Argonaute 1 protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ile392 of human Argonaute 1 protein, Val160 of human Argonaute 3 protein, and corresponding to mouse Argonaute 2 and residues near the amino terminus of human Argonaute 4 protein.

Background

Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4), and Piwi-interacting RNAs (piRNAs) (5), have been identified. miRNAs are about 21 nucleotides in length and have been implicated in many cellular processes, such as development, differentiation, and stress response (1,2). miRNAs regulate gene expression by modulating mRNA translation or stability (2). miRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1,2). There are four members in the mammalian Argonaute family, and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2). Argonaute proteins participate in the various steps of miRNA-mediated gene silencing, such as repression of translation and mRNA turnover (1).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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