AGK Antibody #58662
Inquiry Info. # 58662
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Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 47 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
AGK Antibody recognizes endogenous levels of total AGK protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AGK protein. Antibodies are purified by peptide affinity chromatography.
Background
Acylglycerol kinase (AGK) is a mitochondrial lipid kinase that phosphorylates monoacylglycerol and diacylglycerol to generate bioactive phospholipids lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively (1, 2). Research studies show that the kinase activity of AGK is required for the activation of PI3K-mTOR signaling and the subsequent enhancement of glycolysis in activated CD8+ T cells. AGK is therefore essential for the growth, proliferation and antitumor function of activated CD8+ T cells (3). In addition, AGK is also a subunit of the mitochondrial TIM22 protein import complex independent of its kinase activity. AGK is critical for the stabilization of the TIM22 complex (2, 4).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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