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  3. Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3115)
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Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3115) #61371

Filter:
  • SCA

    Product Specifications

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • SCA-Single Cell Analysis 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Storage

    Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (InTraSeq™ 3' Conjugate 3115) recognizes endogenous β-catenin protein when residues Ser33, Ser37, and Thr41 are not phosphorylated. It does not detect β-catenin protein if tri-phosphorylated at Ser33/Ser37/Thr41. This antibody may also detect β-catenin protein when singly phosphorylated at Ser33.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser37 of human β-catenin protein.

    Background

    β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
    Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb is designed to specifically recognize the stabilized form of β-catenin, i.e., protein that has not been phosphorylated by GSK-3, and thus is functionally active in cell-cell adhesion and/or the canonical Wnt signaling pathway.

    Alternate Names

    armadillo; Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta 1; Catenin beta-1; CTNB1; CTNNB; CTNNB1; DKFZp686D02253; EVR7; FLJ25606; FLJ37923; MRD19; NEDSDV

    Database Links

    UniProt ID: P35222


    Entrez-Gene Id: 1499


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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