PathScan® Sandwich ELISA Control Phospho Cell Extract II #7989
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Product Information
Storage
Supplied as lyophilized product. Keep at -20°C for short-term storage or at -80°C for longer periods. Avoid repeated freeze-thaw cycles.
Product Description
Jurkat cells are treated with 100 nM calyculin A and 1 mM pervanadate to inhibit multiple serine/threonine and tyrosine phosphatases, respectively and upregulate protein phosphorylation. Treated Jurkat cells were lysed in 1X cell lysis buffer and lysates were lyophilized.
Background
PathScan® Sandwich ELISA is a convenient tool for the analysis of protein expression as well as post-translational modifications (such as phosphorylation and acetylation) allowing the researcher to study signal transduction. PathScan® Sandwich ELISA Control Phospho Cell Extracts II can be used not only to troubleshoot the experiment but also provides the means to standardize the signal allowing the comparison of results obtained from different plates or experiments.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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