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PathScan^® Total IRS-2 Sandwich ELISA Antibody Pair

PathScan^® Total IRS-2 Sandwich ELISA Antibody Pair #7885

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Inquiry Info. # 7885

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Product Specifications

REACTIVITY

Application Key:

ELISA+-ELISA and/or ELISA-like Assays

Species Cross-Reactivity Key:

M-Mouse

Product Information

Storage

Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.

实验步骤

Product Description

CST's PathScan^® Total IRS-2 Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan^® Total IRS-2 Sandwich ELISA Kit #7884. Capture and detection antibodies (100X stocks) and HRP-conjugated streptavidin (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IRS-2 rabbit capture antibody is coated in PBS overnight onto a 96 well microplate. After blocking, cell lysate is added followed by an IRS-2 biotinylated rabbit detection antibody and HRP-linked streptavidin. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-2.

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan^® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Mouse

Background

Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).

Alternate Names

diabetes; insulin; insulin receptor; insulin receptor substrate; insulin receptor substrate-2; IRS; IRS-2; IRS2; rtkscaffold; sandwich ELISA; screening

Database Links

UniProt ID: Q9Y4H2

Entrez-Gene Id: 8660

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For Research Use Only. Not for Use in Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a registered trademark of Cell Signaling Technology, Inc.

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PathScan® Total IRS-2 Sandwich ELISA Antibody Pair #7885