PathScan® Total IKK alpha Sandwich ELISA Kit

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Product Specifications

REACTIVITY

H

Application Key:

ELISA+-ELISA and/or ELISA-like Assays

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

The PathScan^® Total IKKα Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of IKKα. An IKKα rabbit antibody has been coated onto the microwells. After incubation with cell lysates, IKKα (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, an IKKα mouse detection mAb is added to the captured phospho and nonphospho IKKα protein. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total IKKα.
Antibodies in kit are custom formulations specific to kit.

实验步骤

Specificity / Sensitivity

The PathScan^® Total IKK alpha Sandwich ELISA Kit detects endogenous levels of total IKKα protein, as shown in Figure 1. This kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).

Alternate Names

CHUK; component of inhibitor of nuclear factor kappa B kinase complex; conserved helix-loop ubiquitous kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; IkappaB kinase; IkB kinase alpha subunit; IkBKA; IKK-A; IKK-a kinase; IKK-alpha; IKK1; IKKA; Inhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NFkappaB inhibitor kinase alpha; TCF-16; TCF16; Transcription factor 16

Database Links

UniProt ID: O15111

Entrez-Gene Id: 1147

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For Research Use Only. Not for Use in Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a registered trademark of Cell Signaling Technology, Inc.

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PathScan® Total IKK alpha Sandwich ELISA Kit #7078