PathScan® RP Rad51 Sandwich ELISA Kit #71823
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Important Ordering Details
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Supporting Data
| REACTIVITY | H M R Mk |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Description
The rapid protocol (RP) PathScan® RP Rad51 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Rad51 protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with Rad51 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of Rad51 protein. Learn more about your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to kit.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
The PathScan® RP Rad51 Sandwich ELISA Kit detects endogenous levels of Rad51 protein. The kit sensitivity is shown in Figures 1 and 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Human, Mouse, Rat, Monkey
Background
DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells sense and repair DSBs via two distinct but partly overlapping signaling pathways: nonhomologous end joining (NHEJ) and homologous recombination (HR). Research studies have shown that defects in both pathways are associated with human diseases, including cancer (reviewed in 1).
DSBs that arise during S or G2 phase are repaired via homologous recombination, using the replicated sister chromatid as a repair template. Rad51 recombinase, a eukaryotic homolog of E. coli RecA, polymerizes and forms a filament along single-stranded DNA, mediating HR with the help of auxiliary proteins, including Rad54 and BRCA2 (reviewed in 2,3). BRCA2 binds Rad51 and targets it to single-stranded DNA, allowing it to displace replication protein A (RPA) (4). Five Rad51 paralogs exist in vertebrates (XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D), and they all appear to be required for efficient HR (5).
Researchers have found that mutations in the Rad51 gene may be related to breast cancer risk (6). Some studies have implicated Rad51 as a potential marker for pancreatic cancer (7).
DSBs that arise during S or G2 phase are repaired via homologous recombination, using the replicated sister chromatid as a repair template. Rad51 recombinase, a eukaryotic homolog of E. coli RecA, polymerizes and forms a filament along single-stranded DNA, mediating HR with the help of auxiliary proteins, including Rad54 and BRCA2 (reviewed in 2,3). BRCA2 binds Rad51 and targets it to single-stranded DNA, allowing it to displace replication protein A (RPA) (4). Five Rad51 paralogs exist in vertebrates (XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D), and they all appear to be required for efficient HR (5).
Researchers have found that mutations in the Rad51 gene may be related to breast cancer risk (6). Some studies have implicated Rad51 as a potential marker for pancreatic cancer (7).
- Hartlerode, A.J. and Scully, R. (2009) Biochem J 423, 157-68.
- Sung, P. et al. (2003) J Biol Chem 278, 42729-32.
- Mazin, A.V. et al. (2010) DNA Repair (Amst) 9, 286-302.
- Jensen, R.B. et al. (2010) Nature 467, 678-83.
- Takata, M. et al. (2001) Mol Cell Biol 21, 2858-66.
- Antoniou, A.C. et al. (2007) Am J Hum Genet 81, 1186-200.
- Nagathihalli, N.S. and Nagaraju, G. (2011) Biochim Biophys Acta 1816, 209-18.
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For Research Use Only. Not for Use in Diagnostic Procedures.
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