PathScan® Phospho-Zap-70 (Tyr319) Sandwich ELISA Antibody Pair #7852
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Inquiry Info. # 7852
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Product Specifications
| REACTIVITY | H |
Application Key:
- ELISA+-ELISA and/or ELISA-like Assays
Species Cross-Reactivity Key:
- H-Human
Product Information
Storage
Capture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.
实验步骤
Product Description
CST's PathScan® Phospho-Zap-70 (Tyr317) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-Zap-70 (Tyr317) Sandwich ELISA Kit #7171. Capture and detection antibodies (100X stocks) and HRP-linked secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The Zap-70 capture antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a Phospho-Zap-70 (Tyr317) detection antibody and anti-Rabbit IgG, HRP-linked antibody. HRP substrate TMB is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho- Zap-70 (Tyr317) protein.
Specificity / Sensitivity
For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Human
Background
The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).
- Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
- Iwashima, M. et al. (1994) Science 263, 1136-9.
- Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.
- Chan, A.C. et al. (1995) EMBO J 14, 2499-508.
- Williams, B.L. et al. (1999) EMBO J 18, 1832-44.
- Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.
- Wiestner, A. et al. (2003) Blood 101, 4944-51.
- Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.
Alternate Names
cell based assay; cell-based assay; sandwich elisa; screening; zap-70 elisa
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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