Name: Mouse G-CSF Recombinant Protein
Price: 245.00 USD
Availability: InStock

Product Information

Product Usage Information

Working concentration of recombinant mG-CSF generally ranges from 10 pg/mL to 100 ng/ml.

Storage

Stable in lyophilized state at -20°C for 24 months after receipt.

It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

Product Description

MW (kDa)	19.1
Purity	A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin	Endotoxin levels are less than or equal to 1 EU / 1 μg mG-CSF.
Activity	The bioactivity of recombinant mG-CSF was determined in an NFS-60 cell proliferation assay. The ED₅₀ of each lot is less than or equal to 50 pg/mL.

Source / Purification

Recombinant mouse G-CSF was expressed in E. coli and is supplied in a lyophilized form.

Background

G-CSF is a hematopoietic cytokine essential for neutrophil development, survival, and egress from bone marrow (1-4). Macrophages and monocytes are the predominant producers of G-CSF (3) and endothelial cells, fibroblasts, and neuronal cells can produce G-CSF in response to inflammatory stimuli (3). G-CSF inhibits apoptosis in neutrophils and neurons (4,5). G-CSF stimulates proliferation and differentiation of neuronal progenitor cells (5). G-CSF binding to G-CSFR induces receptor dimerization and activation of Jak1/2 tyrosine phosphorylation (3,6). Signaling is through Stat3, ERK, p38, and Akt (5,6). Absence of functional G-CSF or its receptor in humans and mice causes neutropenia (7,8).

Database Links

UniProt ID: P09920

Entrez-Gene Id: 12985

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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Mouse G-CSF Recombinant Protein #73109