HTScan® Pim-1 Kinase Assay Kit

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Product Information

Product Description

The kit provides a means of performing kinase activity assays with recombinant human Pim-1 kinase. It includes active Pim-1 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-serine/threonine antibody for detection of the phosphorylated form of the substrate peptide.

Molecular Formula

Peptide substrate, Biotin-Bad (Ser112): 1,700 Daltons. GST-Pim-1 Kinase: 65 kDa.

Background

Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12).

Alternate Names

enzyme; high throughput; HTS; kinase assay; PIM; Proto-oncogene serine/threonine-protein kinase pim-1; screening; serine/threonine kinase

Database Links

UniProt ID: P11309

Entrez-Gene Id: 5292

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For Research Use Only. Not for Use in Diagnostic Procedures.

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HTScan® Pim-1 Kinase Assay Kit #7573