FluoroClear Blocking Buffer #33449
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Product Usage Information
Add 5 μL of FluoroClear Blocking Buffer to cell suspension in Antibody Dilution Buffer (may be purchased as ready-to-use Flow Cytometry Antibody Dilution Buffer #13616 or prepared as a solution of 0.5% Bovine Serum Albumin in 1X Phosphate Buffered Saline) prior to addition of antibody conjugates. Final volume of cell suspension, FluoroClear Blocking Buffer, and antibody mixture should equal 100 μL. Post antibody staining, perform washes with Antibody Dilution Buffer and proceed with flow cytometric analysis.
Storage
Supplied in Phosphate Buffered Saline. Store at 4°C. Do not freeze.
Product Description
FluoroClear Blocking Buffer is a non-antibody based blocking buffer designed to reduce non-specific background associated with fluorescent dye conjugated antibodies in fixed/permeabilized cell preparations. This buffer can reduce background associated with multiple dye types, including Alexa Fluor®, mFluor™, Pacific Blue™, and protein dyes such as PE, PE-Cy®7, APC, and APC-Cy®7 without impacting specific antibody staining. Buffer effectiveness varies by specific dye and cell type.
FluoroClear Blocking Buffer is compatible with Cell Signaling Technology® flow cytometry protocols.
FluoroClear Blocking Buffer is compatible with Cell Signaling Technology® flow cytometry protocols.
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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