Whitlow/218 Linker (1B4A1) Mouse Monoclonal Antibody (PE Conjugate) #94779
Filter:
- F
Product Specifications
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Mouse IgG2a |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to phycoerythrin (PE) fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same reactivity as the unconjugated Whitlow/218 Linker (1B4A1) Mouse mAb #28307, which is expected to react with cell surface expressed CARs of varying specificity containing a Whitlow/218 linker within the scFv of the extracellular domain.
Fluorescent Properties
- ← Excitation: 565 nm ← Emission: 575 nm
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
| Flow Cytometry (Live) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
实验步骤
Specificity / Sensitivity
Whitlow/218 Linker (1B4A1) Mouse Monoclonal Antibody (PE Conjugate) recognizes exogenously expressed levels of scFv-based CARs containing a Whitlow/218 linker.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow/218 linker peptide.
Background
The Whitlow/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1).
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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