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PGLYRP-1/PGRP-S (E3B6M) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate) #71841
Filter:
- F
Product Specifications
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated PGLYRP-1/PGRP-S (E3B6M) Rabbit Monoclonal Antibody #19360. To reduce dye-related nonspecific background associated with fluorescent dye-conjugated antibodies in fixed/permeabilized cell preparations, we recommend using FluoroClear Blocking Buffer #33449 in conjunction with PGLYRP-1/PGRP-S (E3B6M) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate).
Fluorescent Properties
- ← Excitation: 495 nm ← Emission: 519 nm
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
实验步骤
Specificity / Sensitivity
PGLYRP-1/PGRP-S (E3B6M) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total PGLYRP-1/PGRP-S protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PGLYRP-1/PGRP-S protein.
Background
The human peptidoglycan recognition protein (PGRP) family is composed of four independent genes named for the relative length of their translated product. Human PGRP family proteins are soluble pattern-recognition receptors of the innate immune response that bind peptidoglycan and Gram-positive bacteria, and have direct bactericidal activity (1,2). PGRP-short (PGRP-S), also known as PGLYRP-1 and Tag7, is 196 amino acids (aa) in length (3). PGLYRP-1/PGRP-S is a secreted glycoprotein expressed by neutrophils that participates in killing phagocytized bacteria (4,5). PGLYRP-1/PGRP-S also has been shown to form a stable cytotoxic complex with the heat shock protein Hsp70 to induce tumor cell death (6,7). PGRP-long (PGRP-L), also known as PGLYRP-2, is 576 aa long. PGLYRP-2/PGRP-L is an N-acetylmuramoyl-L-alanine amidase that hydrolyzes bacterial peptidoglycan (8,9). PGRP-intermediate alpha, also known as PGLYRP-3, and PGRP-intermediate beta, also known as PGLYRP-4, are 341 aa and 373 aa long, respectively. PGLYRP-3 and PGLYRP-4 are co-expressed in the same cells and are exclusively secreted as homo- or heterodimers with antibacterial function (10).
- Dziarski, R. and Gupta, D. (2006) Cell Microbiol 8, 1059-69.
- Liu, C. et al. (2001) J Biol Chem 276, 34686-94.
- Kang, D. et al. (1998) Proc Natl Acad Sci U S A 95, 10078-82.
- Liu, C. et al. (2000) J Biol Chem 275, 24490-9.
- Dziarski, R. et al. (2003) Blood 102, 689-97.
- Yashin, D.V. et al. (2015) J Biol Chem 290, 21724-31.
- Yashin, D.V. et al. (2016) Biochimie 123, 32-6.
- Gelius, E. et al. (2003) Biochem Biophys Res Commun 306, 988-94.
- Wang, Z.M. et al. (2003) J Biol Chem 278, 49044-52.
- Lu, X. et al. (2006) J Biol Chem 281, 5895-907.
Alternate Names
MGC126894; MGC126896; Peptidoglycan recognition protein 1; Peptidoglycan recognition protein short; PGLYRP; PGLYRP1; PGRP; PGRP-S; PGRP1; PGRPS; TAG7; TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein); TNFSF3L
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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