P2A/T2A (3H4-3A5) Mouse Monoclonal Antibody (Alexa Fluor® 488 Conjugate) #24075
Filter:
- F
Product Specifications
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated P2A/T2A (3H4-3A5) Mouse Monoclonal Antibody #72901.
Fluorescent Properties
- ← Excitation: 495 nm ← Emission: 519 nm
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
实验步骤
Specificity / Sensitivity
P2A/T2A (3H4-3A5) Mouse Monoclonal Antibody (Alexa Fluor® 488 Conjugate) recognizes exogenously expressed levels of P2A/T2A peptide motif. Many antibodies are manufactured using P2A/T2A expression, so if running in a panel we recommend testing first for cross-reactivity. Nonspecific centrosome staining may be observed by immunofluorescence.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of a T2A peptide motif.
Background
2A peptides are viral oligopeptides between 18-22 amino acids long that mediate the polypeptide cleavage during translation in eukaryotic cells. They have been developed for genetic engineering applications to enable the simultaneous expression of multiple genes from a single construct (1,2). The first 2A peptide discovered was foot-and-mouth disease virus (F2A), followed by equine rhinitis A virus (E2A), porcine teschovirus-1 (P2A), and thosea asigna virus (T2A) (3). A conserved sequence is shared across the 2As at the C-terminus, where self-cleavage occurs via ribosomal skipping of a glycyl-prolyl peptide bond (4,5).
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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