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p27 Kip1 (D69C12) Rabbit Monoclonal Antibody (Biotinylated)

p27 Kip1 (D69C12) Rabbit Monoclonal Antibody (Biotinylated) #13715

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Product Specifications

REACTIVITY	H R Mk
SENSITIVITY	Endogenous
MW (kDa)	27
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
R-Rat
Mk-Monkey

Product Information

Product Description

This Cell Signaling Technology^® antibody is the biotinylated version of the unconjugated p27 Kip1 (D69C12) Rabbit Monoclonal Antibody #3686 and is expected to exhibit the same species cross-reactivity. The concentration of the biotinylated antibody is 500 ug/ml. Peptide ELISA data were generated using the biotinylated antibody. Western blot data were generated using the unconjugated antibody.

MW (kDa)

Product Usage Information

Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET.

Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution.

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

p27 Kip1 (D69C12) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total p27 Kip1 protein.

Species Reactivity:

Human, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human p27 Kip1 protein.

Background

p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).

Alternate Names

CDKN1B; CDKN4; CDN1B; cyclin dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; KIP1; MEN1B; MEN4; p27Kip1

Database Links

UniProt ID: P46527

Entrez-Gene Id: 1027

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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