MHC Class II (I-A/I-E) (M5/114.15.2) Rat Monoclonal Antibody (FITC Conjugate) #42594
- IF
- F
Product Specifications
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rat IgG2b kappa |
Application Key:
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:200 - 1:800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:200 |
| Flow Cytometry (Live) | 1:200 |
Storage
实验步骤
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
The M5/114.15.2 antibody reacts with mouse MHC class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes, but not from mice carrying the H-2s or H-2f haplotypes (4-7). The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, and H-2u haplotypes, but not H-2f, H-2k, or H-2s haplotypes (8,9).
- Ting, J.P. and Trowsdale, J. (2002) Cell 109 Suppl, S21-33.
- Cresswell, P. (1994) Annu Rev Immunol 12, 259-93.
- Karp, D.R. et al. (1990) J Exp Med 171, 615-28.
- Staehli, F. et al. (2012) J Immunol 188, 3820-8.
- Scarlett, U.K. et al. (2012) J Exp Med 209, 495-506.
- Anderson, M.S. and Miller, J. (1992) Proc Natl Acad Sci U S A 89, 2282-6.
- Miyazaki, T. et al. (1996) Cell 84, 531-41.
- Parra, D. et al. (2012) J Leukoc Biol 91, 525-36.
- Chen, M. et al. (2011) J Immunol 187, 5684-92.
Alternate Names
A alpha; A beta; A130038H09Rik; Aalpha; Abe; Abeta; AI845868; cell surface glycoprotein; E-beta-b; E-W17; H-2; H-2 class II histocompatibility antigen E-a/k beta-2; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, A-B alpha chain; H-2 class II histocompatibility antigen, A-D alpha chain; H-2 class II histocompatibility antigen, A-D beta chain; H-2 class II histocompatibility antigen, A-F alpha chain; H-2 class II histocompatibility antigen, A-F beta chain; H-2 class II histocompatibility antigen, A-K alpha chain; H-2 class II histocompatibility antigen, A-K beta chain; H-2 class II histocompatibility antigen, A-Q alpha chain; H-2 class II histocompatibility antigen, A-Q beta chain; H-2 class II histocompatibility antigen, A-R alpha chain; H-2 class II histocompatibility antigen, A-S alpha chain; H-2 class II histocompatibility antigen, A-S beta chain; H-2 class II histocompatibility antigen, A-U alpha chain; H-2 class II histocompatibility antigen, A-U beta chain; H-2 class II histocompatibility antigen, E-B beta chain; H-2 class II histocompatibility antigen, E-D beta chain; H-2 class II histocompatibility antigen, E-Q beta chain; H-2 class II histocompatibility antigen, E-S beta chain; H-2 class II histocompatibility antigen, I-A beta chain; H-2 class II histocompatibility antigen, I-E alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; H-2 I-A beta p; H-2A; H-2Aa; H-2Ab; H-2E beta; H-2Eb; H-2Eb2; H2-A; H2-Aa; H2-Ab; H2-Ab_; H2-Ab1; H2-Eb_p; H2-Eb1; H2-Eb2; H2Aa; H2Eb; HA2F; HB2I; HB2Q; histocompatibility 2, class II antigen A, alpha; histocompatibility 2, class II antigen A, alpha, promoter 1; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen A, beta, promoter 1; histocompatibility 2, class II antigen E beta; histocompatibility 2, class II antigen E beta, promoter 1; Histocompatibility 2, class II antigen E beta2; HLA-DQA1; HLA-DQB1; HLA-DRB1; I-A beta MHC class II; I-A<; I-A<b>; I-A<SUP>b</SUP>; I-Aalpha; I-Ab; I-Abeta; I-E beta MHC class II; I-E beta-chain; Ia-1; Ia-2; Ia-4; Ia-5; Ia1; Ia2; Ia4; Ia5; IAalpha; IAb; Iebeta2; lymphocyte antigen; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; major histocompatibility complex H-2E beta chain'; major histocompatibility protein class II alpha chain; MGC163794; MGC163796; MHC class II antigen; MHC class II antigen A beta; MHC class II antigen alpha chain; MHC class II antigen beta chain; MHC class II antigen E beta2; MHC class II beta chain A-beta-5; MHC class II beta chain A-beta-7; MHC class II E beta; MHC class II E-beta-2; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC class II protein; MHC E-beta-f; MHC H2-IA-beta-1 (bm12) chain; MHC H2-IE-beta cell surface glycoprotein; MHC II H2-IE-beta; MHC-II I-A(d) alpha; MHC-II I-A(d) beta; response to metastatic cancers 1; Rmc; Rmcs1
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