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MDM2 (D1V2Z) Rabbit Monoclonal Antibody (Biotinylated)

MDM2 (D1V2Z) Rabbit Monoclonal Antibody (Biotinylated) #76794

Filter:

WB
ELISA+

Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	90
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
ELISA+-ELISA and/or ELISA-like Assays

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

This Cell Signaling Technology^® antibody is the biotinylated version of the unconjugated MDM2 (D1V2Z) Rabbit Monoclonal Antibody #86934 and is expected to exhibit the same species cross-reactivity. The concentration of the biotinylated antibody is 500 ug/ml. Peptide ELISA data were generated using the biotinylated antibody. Western blot data were generated using the unconjugated antibody.

MW (kDa)

Product Usage Information

Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET.

Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution.

Storage

Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

MDM2 (D1V2Z) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total MDM2 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect full-length MDM2, as well as isoforms A, C, alpha, F, G, and 11. This antibody may detect isoform A1.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val280 of human MDM2 protein.

Background

MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3).

Alternate Names

ACTFS; Double minute 2 protein; double minute 2, human homolog of; E3 ubiquitin-protein ligase Mdm2; Hdm2; HDMX; LSKB; MDM2; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2 p53 binding protein homolog (mouse); MDM2 proto-oncogene; MDM2 proto-oncogene, E3 ubiquitin protein ligase; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; Oncoprotein Mdm2; p53-binding protein; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2; ubiquitin-protein ligase E3 Mdm2

Database Links

UniProt ID: Q00987

Entrez-Gene Id: 4193

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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