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CCR4 (F9A9K) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate) #42346
Filter:
- IHC
Product Specifications
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated CCR4 (F9A9K) Rabbit mAb #59945.
Fluorescent Properties
- ← Excitation: 495 nm ← Emission: 519 nm
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
实验步骤
Specificity / Sensitivity
CCR4 (F9A9K) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total CCR4 protein. This antibody detects a 15-20 kDa protein of unknown identity in some cell lines.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CCR4 protein.
Background
C-C chemokine receptor type 4 (CCR4) is a chemokine receptor that is primarily expressed in T cells, specifically in Th2, Th17, and Treg subsets (1). Like other chemokine receptors, CCR4 is a G protein-coupled receptor (GPCR) that contains seven transmembrane domains (2). CCR4 selectively binds with the chemokine ligands CCL17, also known as thymus and activation-related chemokine (TARC), and CCL22, also known as macrophage-derived chemokine (MDC). CCL17/TARC functions as a chemoattractant for CCR4-expressing T cells and is associated with type 2 immune responses (3,4). CCL22/MDC is expressed by macrophages and dendritic cells and also functions as a chemoattractant with dominant binding to CCR4 compared with CCL17/TARC (5-7). Elevated CCR4 expression has been implicated in various allergic diseases, such as asthma and dermatitis (8,9). It has also been implicated in several hematological malignancies, including acute myeloid leukemia (AML), acute T cell leukemia/lymphoma (ATLL), and cutaneous T cell lymphoma (CTLL) (1,10). The humanized anti-CCR4 monoclonal antibody mogamulizumab has been approved for the treatment of patients with relapsed ATLL (11).
- Yoshie, O. (2021) Cancers (Basel) 13, 5542. doi: 10.3390/cancers13215542.
- Solari, R. and Pease, J.E. (2015) Eur J Pharmacol 763, 169-77.
- Imai, T. et al. (1996) J Biol Chem 271, 21514-21.
- Islam, S.A. and Luster, A.D. (2012) Nat Med 18, 705-15.
- Imai, T. et al. (1998) J Biol Chem 273, 1764-8.
- Mariani, M. et al. (2004) Eur J Immunol 34, 231-40.
- Viney, J.M. et al. (2014) J Immunol 192, 3419-27.
- Perros, F. et al. (2009) Allergy 64, 995-1002.
- Smith, J.S. et al. (2018) Dermatitis 29, 179-186.
- Zou, S. et al. (2024) Discov Oncol 15, 412.
- Ureshino, H. et al. (2019) Clin Lymphoma Myeloma Leuk 19, 326-331.
Alternate Names
C-C chemokine receptor type 4; C-C CKR-4; C-C motif chemokine receptor 4; CC-CKR-4; CCR-4; CCR4; CD194; chemokine (C-C motif) receptor 4; chemokine (C-C) receptor 4; ChemR13; CKR4; CMKBR4; HGCN:14099; K5-5; MGC88293
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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