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  3. Ionomycin, Calcium Salt

Ionomycin, Calcium Salt #9995

    Product Information

    Product Usage Information

    Ionomycin, calcium salt is supplied as 2.5 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1.1 ml DMSO to make a 3 mM ionomycin stock solution. For working concentrations of 1 μM-3 μM, dilute DMSO stock 1:3000 to 1:1000. Treat cells with the desired concentration for intended time.

    Storage

    Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

    Product Description

    Molecular Weight 747.08 g/mol
    Purity >99%
    Molecular Formula C41H70O9 • Ca2+
    CAS 56092-82-1
    Solubility Soluble in DMSO at 1.6mg/ml and EtOH at 20mg/ml.

    Background

    Ionomycin is a potent and selective calcium ionophore agent (1,2). The molecule acts as a motile Ca2+ carrier and enhances Ca2+ influx by direct stimulation of store-regulated cation entry across biological membranes (3). At the micromolar level, ionomycin can activate Ca2+/Calmodulin dependent kinase and phosphatase to stimulate gene expression (4). In human T cells, ionomycin induces hydrolysis of phosphoinositides and activates PKC to mediate T cell activation (5). Ionomycin treatment of human B cells induces the activation of calcium-dependent endonuclease and results in apoptosis (6). Ionomycin treatment of bovine aortic endothelial cells (BAECs) induces rapid dephosphorylation of eNOS at Thr495 and mediates eNOS activation (7).

    Alternate Names

    BAECs; bovine aortic endothelial cells; calcium carrier; calcium ionphore agent; calmodulin; eNOS; PKC

    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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