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Etoposide #2200

    Product Information

    Product Usage Information

    Etoposide is supplied as a lyophilized powder. For a 25 mM stock, reconstitute the 5.9 mg in 400 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µM for 4-24 hr. Soluble in DMSO at 25 mg/ml; very poorly soluble in ethanol and water.

    Wear personal protective equipment. Do not handle until all safety precautions have been read and understood.

    Safety Information: Etoposide has been classified by the International Agency for Research on Cancer (IARC) as a known human carcinogen (Group 1). It may cause adverse reproductive effects - such as birth defect, miscarriages, or infertility. Avoid contact during pregnancy and while nursing. If exposed or concerned, get medical advice. See Safety Data Sheet (SDS).

    Storage

    Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

    Product Description

    Molecular Weight 588.56 g/mol
    Purity >98%
    Molecular Formula C29H32O13
    CAS 33419-42-0
    Solubility Soluble in DMSO at 25mg/ml.

    Background

    An anti-tumor agent that is commonly used as an apoptosis inducer, etoposide (VP-16) is a topoisomerase II inhibitor with an IC50 of 59.2 µM (1). Etoposide stabilizes a covalent topoisomerase II-cleaved DNA intermediate complex in the catalytic cycle of the enzyme, leading to genomic instability and cell death (2,3). This mechanism of action has been shown to delay progression of the cell cycle through the late S and early G2 phase (4,5).

    Alternate Names

    anti-tumor; apoptosis; cleaved parp; covalent topoisomerase II-cleaved DNA; VP-16

    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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