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NCR1/NKp46 (F8M2L) Rabbit Monoclonal Antibody (BSA and Azide Free) #71583
Filter:
- WB
- IHC
- F
Product Specifications
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50-60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
This product is the carrier free version of products #10936 and #71888. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see products #10936 and #71888.
For standard formulation of this product see products #10936 and #71888.
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
NCR1/NKp46 (F8M2L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total NCR1/NKp46 protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse NCR1/NKp46 protein.
Background
The family of natural cytotoxicity receptors (NCRs) contains NCR1 (NKp46/CD335), NCR2 (NKp44/CD336), and NCR3 (NKp30/CD337). They are single-pass type I transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. Various pathogen-derived and host-encoded molecules have been identified as ligands for the NCRs. They were originally identified by their ability to evoke the cytotoxicity of natural killer (NK) cells toward tumor cells in vitro, and animal models have demonstrated that NCRs play a role in vivo in tumor surveillance, viral infection, and pregnancy (1-5).
NCR1/NKp46 is considered a universal marker for NK cells, and recent studies found that it is also expressed by other cells, such as group 1 innate lymphoid cells (ILC1), a subset of group 3 ILCs (NCR+ ILC3), and γδ T cells (2-4). NCR1/NKp46 is also expressed on and considered a diagnostic marker and therapeutic target for some malignant NK, NKT, and T cell lymphomas (6,7). Cross-linking NCR1/NKp46 with antibodies can activate NK cells, and this has been pursued as a promising therapeutic avenue (8).
NCR1/NKp46 is considered a universal marker for NK cells, and recent studies found that it is also expressed by other cells, such as group 1 innate lymphoid cells (ILC1), a subset of group 3 ILCs (NCR+ ILC3), and γδ T cells (2-4). NCR1/NKp46 is also expressed on and considered a diagnostic marker and therapeutic target for some malignant NK, NKT, and T cell lymphomas (6,7). Cross-linking NCR1/NKp46 with antibodies can activate NK cells, and this has been pursued as a promising therapeutic avenue (8).
- Sivori, S. et al. (1997) J Exp Med 186, 1129-36.
- Kruse, P.H. et al. (2014) Immunol Cell Biol 92, 221-9.
- Pazina, T. et al. (2017) Front Immunol 8, 369.
- Barrow, A.D. et al. (2019) Front Immunol 10, 909.
- Meza Guzman, L.G. et al. (2020) Cancers (Basel) 12, 952.
- Laribi, K. et al. (2018) Recent Pat Anticancer Drug Discov 13, 308-340.
- Cheminant, M. et al. (2019) Gut 68, 1396-1405.
- Gauthier, L. et al. (2019) Cell 177, 1701-1713.e16.
Alternate Names
Activating receptor 1; CD335; Ly9; Ly94; Lymphocyte antigen 94; mAR-1; MAR1 (mouse activating receptor 1); mNKp46; Natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NCTR1; NK-p46; NKp; NKp46
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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