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Mre11 Antibody

Mre11 Antibody #4895

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Product Specifications

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)	81
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IHC-Immunohistochemistry

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Immunohistochemistry (Paraffin)	1:500

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

实验步骤

Specificity / Sensitivity

Mre11 Antibody detects endogenous levels of Mre11 homologue A (Mre11A). This antibody may cross-react with Mre11 homologue B (Mre11B).

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys496 of human Mre11A. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Mre11, originally described in genetic screens from the yeast Saccharomyces cerevisiae in which mutants were defective in meiotic recombination (1), is a central part of a multisubunit nuclease composed of Mre11, Rad50 and Nbs1 (MRN) (2,3). The MRN complex plays a critical role in sensing, processing and repairing DNA double strand breaks. Defects lead to genomic instability, telomere shortening, aberrant meiosis and hypersensitivity to DNA damage (4). Hypomorphic mutations of Mre11 are found in ataxia-telangiectasia-like disease (ATLD), with phenotypes similar to mutations in ATM that cause ataxia-telangiectasia (A-T), including a predisposition to malignancy in humans (5). Cellular consequences of ATLD include chromosomal instability and defects in the intra-S phase and G2/M checkpoints in response to DNA damage. The MRN complex may directly activate the ATM checkpoint kinase at DNA breaks (6).

Alternate Names

AT-like disease; ATLD; DNA recombination and repair protein; Double-strand break repair protein MRE11; Double-strand break repair protein MRE11A; endo/exonuclease Mre11; HNGS1; Meiotic recombination 11 homolog 1; Meiotic recombination 11 homolog A; MRE11; MRE11 double strand break repair nuclease A; MRE11 homolog 1; MRE11 homolog A; MRE11 homolog A, double strand break repair nuclease; MRE11 homolog, double strand break repair nuclease; MRE11 homolog, double strand break repair nuclease A; MRE11 meiotic recombination 11 homolog A; MRE11 meiotic recombination 11 homolog A (S. cerevisiae); MRE11 meiotic recombination 11-like protein A; MRE11A; MRE11B

Database Links

UniProt ID: P49959

Entrez-Gene Id: 4361

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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