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  3. Phospho-eEF2k (Ser366) Antibody

Phospho-eEF2k (Ser366) Antibody #3691

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    Product Specifications

    REACTIVITY H R Mk
    SENSITIVITY Endogenous
    MW (kDa) 105
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-eEF2k (Ser366) Antibody detects endogenous levels of eEF2k only when phosphorylated at Ser366.

    Species Reactivity:

    Human, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser366 of human eEF2k. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Eukaryotic elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation (1). eEF2k is normally dependent on Ca2+ ions and calmodulin (2,3). It can be activated by PKA in response to elevated cAMP levels (4-6), which are generally increased in stress- or starvation-related conditions. eEF2k can also be regulated in response to a wide range of stimuli that promote cell growth and protein synthesis. This involves the phosphorylation of eEF2k by p90RSK and p70 S6 kinase at Ser366 or by SAPK4/p38delta at Ser359, leading to the inactivation of eEF2k (7,8), which facilitates the dephosphorylation of eEF2, and thus promotes translation.

    Alternate Names

    alternative protein EEF2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; CaMKIII; eEF-2 kinase; eEF-2K; EEF2K; EF2K; elongation factor 2 kinase; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; HSU93850; MGC45041

    Database Links

    UniProt ID: O00418


    Entrez-Gene Id: 29904


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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