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  3. Phospho-FAK (Tyr397) Antibody

Phospho-FAK (Tyr397) Antibody #3283

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  • WB

    Product Specifications

    REACTIVITY H M R Hm Pg
    SENSITIVITY Endogenous
    MW (kDa) 125
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 
    • Pg-Pig 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-FAK (Tyr397) Antibody detects endogenous levels of FAK only when phosphorylated at Tyr397. This antibody may cross-reacts with other tyrosine-phosphorylated RTKs.

    Species Reactivity:

    Human, Mouse, Rat, Hamster, Pig

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human FAK. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).

    Alternate Names

    FADK; FADK 1; FAK; FAK-related non-kinase polypeptide; FAK1; Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; FRNK; p125FAK; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein tyrosine kinase 2; Protein-tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2

    Database Links

    UniProt ID: Q05397


    Entrez-Gene Id: 5747


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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