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Phospho-alpha-Synuclein (Ser129) (D1R1R) Rabbit Monoclonal Antibody

Phospho-alpha-Synuclein (Ser129) (D1R1R) Rabbit Monoclonal Antibody #23706

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Product Specifications

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	18
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IHC-Immunohistochemistry
IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:400 - 1:1600
Immunofluorescence (Frozen)	1:400 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier-free (BSA and azide free) version of this product see product #87281.

实验步骤

Specificity / Sensitivity

Phospho-alpha-Synuclein (Ser129) (D1R1R) Rabbit Monoclonal Antibody recognizes endogenous levels of α-Synuclein protein only when phosphorylated at Ser129. Specificity of nuclear staining is unclear but consistent with previous publications (4).
This antibody is recommended for immunohistochemical analysis of human neuronal tissues only. Non-specific staining was observed in non-neuronal human tissues and in mouse tissues.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser129 of human α-Synuclein protein.

Background

α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-Synuclein gene are linked to Parkinson's disease (1).

Various research studies have shown that phosphorylation of α-Synuclein at Ser129 is a highly toxic event causing degeneration of dopaminergic neurons associated with Parkinson's disease. This is proposed to occur through increased misfolding, aggregation, and accumulation of α-Synuclein phosphorylated at this site (2). GSK-3β is one of several kinases that has been reported to phosphorylate α-Synuclein at Ser129 (3).

Alternate Names

Alpha-synuclein; alpha-synuclein, isoform NACP140; I+/--synuclein; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; non-A4 component of amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; SNCA; synuclein alpha; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor); SYUA; truncated alpha synuclein

Database Links

UniProt ID: P37840

Entrez-Gene Id: 6622

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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