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  3. Cleaved alpha-Fodrin (Asp1185) Antibody

Cleaved alpha-Fodrin (Asp1185) Antibody #2121

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 150
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    实验步骤

    Specificity / Sensitivity

    Cleaved alpha-Fodrin (Asp1185) Antibody detects endogenous levels of the large fragment of alpha-fodrin resulting from cleavage at aspartic acid 1185. This antibody does not recognize full length alpha-fodrin.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues surrounding Asp1185 in human alpha-fodrin. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Fodrin (also named nonerythroid spectrin) is a universally expressed membrane-associated cytoskeletal protein consisting of alpha- and beta-subunits (1). This protein is important for maintaining normal membrane structure and supporting cell surface protein function (1). Alpha-fodrin is one of the primary targets cleaved by caspases during apoptosis. The full length 240 kDa protein can be cleaved at several sites within its sequence by activated caspases to yield amino-terminal 150 kDa, carboxy-terminal 120 kDa and 35 kDa major products (2-5). Cleavage of alpha-fodrin leads to membrane malfunction and cell shrinkage.

    Alternate Names

    (ALPHA)II-SPECTRIN; alpha-fodrin; Alpha-II spectrin; EIEE5; epididymis secretory sperm binding protein; FLJ44613; Fodrin alpha chain; NEAS; non-erythrocytic spectrin alpha; Spectrin alpha chain, brain; Spectrin alpha chain, non-erythrocytic 1; spectrin alpha, non-erythrocytic 1; spectrin, alpha, non-erythrocytic 1 (alpha-fodrin); Spectrin, non-erythroid alpha chain; Spectrin, non-erythroid alpha subunit; SPTA2; SPTAN1; SPTN1

    Database Links

    UniProt ID: Q13813


    Entrez-Gene Id: 6709


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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